Animal and Livestock Biotechnology

Animal Biotechnology

Animal Biotechnology programme of the department is focused towards improving animal health by developing newer vaccines and diagnostics, development of newer reproductive technologies genomics and genetic characterization, production of bio-pharmaceuticals through transgenisis and animal products. India has the largest animal husbandry sector in the world with largest livestock population, which supports the livelihoods of more than two-thirds of the rural population, mainly small and marginal farmers. Livestock not only sustain food demands of the rural households but also provides stability at the time of crop failures. Therefore, it is important to ensure sustainable animal production and health. Moreover, healthy animals are closely related to healthy people and healthy environment. Hence, it is also essential to alleviate animal ailments for improved animal health. The main aims of this program are the sustainable growth of livestock and poultry for nutritional security and economic prosperity as well as enhance production and productivity of livestock through biotechnological interventions.

 

Objectives

  • R&D support to develop novel reproductive technologies including developing transgenic and cloned animals
  • To conduct basic and applied research in the area of nutrition using physiological and nutritional approach
  • Genome analysis and genetic characterization of indigenous livestock breeds
  • Development of affordable new generation vaccines and diagnostics against major diseases of livestock, dogs and poultry
  • To train quality human resource and develop infrastructure in animal biotechnology
  • To initiate translational research and product development in animal biotechnology area
  • To generate network programs in the priority areas of animal health and reproduction
  • To implement PAN India program on Canine Health Research

 

New Initiatives:

  1. One Health Program:   A new programme for establishment of a consortium for ‘One Health’ for addressing Zoonoses towards detection and control of select transboundary and zoonotic diseases has been initiated.
  2. Development of novel semen extender to optimize post thaw quality:  To develop novel semen extender of goats with improved post-thaw fertility and conception rate to develop/standardize cryopreservation protocol for goat semen which will help in enhancement of goat productivity.
  3. Para-tuberculosis infection in indigenous breeds of cows:  To identify key molecular factors involved in resistance/susceptibility to para-tuberculosis infection in indigenous breeds of cows.
  4. A programme to enhance the shelf life of an oocyte to increase the fertilization time window:  To enhance the shelf life of an oocyte by targeting the epigenetic modifications in mouse and goat models using siRNA or small molecule inhibitors.
  5. Early detection of pregnancy in buffalo:     Aptamers based diagnosis in the form of nano-biosensors for early detection of pregnancy in buffalo that can bind to pre-selected targets including proteins with high affinity, specificity and selectivity.

 

Significant Highlights and Achievements:

  1. Livestock genetics & Genomics: Whole genome sequencing (30X genomics) of all 43 indigenous cattle breads has been carried out and identified SNPs that would be placed on the chip for further use to delineate the admixture in cattle available in field and organized farms.
  2. Livestock reproduction: To develop an early pregnancy diagnostic for cattle and buffalo various key proteins/ metabolites/multiple antigenic peptides from urine, saliva and serum has been identified which have the potential to detect pregnancy at an early stage.
  3. Production of therapeutics: Goat mammary epithelial cell (gMECs) culture (both primary and secondary) was standardized and transfected in vivo for expressing targeted proteins. The cDNA of therapeutic protein gene, human blood coagulation factor 8 (hF8) and human tissue plasminogen activator (hTPA) have been validated in gMECs culture. Production of cattle gonadotropins in milk of rabbit by in vivo gene transfection has also been carried out and cDNA of alpha subunit of FSH/LH protein has been validated.
  4. Canine Health Research:     Lateral Flow Assay (LFA) kit for diagnosis of Immune mediated haemolytic anaemia and a PCR based diagnostic technology for the detection of Haemoprotozoan parasites have been developed and ready for commercialization. A canine blood bank unit has been established and a total of 195 dog blood samples of different dog breeds have been screened with canine blood typing kit (KABB). The field virus isolates of Canine Distemper Virus (CDV) were serially passaged, scaled-up and whole genome sequencing was carried out to explore suitable vaccine candidate(s).
  5. Translational Research Platform for Veterinary Biologicals (TRPVB):            Two technologies have been commercialized viz virus like particle (VLP) based vaccine for Canine Parvo Virus using baculovirus expression System (BEVS), was transferred to M/s Palamur Biosciences, Hyderabad and technology of PCR based diagnosis of canine haemoprotozan diseases was transferred to M/s. Illume Gene Private Limited. A large  number  of  veterinary  biologicals were also  produced  for  State  Veterinary  department  of  Tamilnadu for  their  distribution  among  farmers.
  6. Mycobacterial diseases in animals’ network (MyDAN):  The synthetic peptides have been evaluated for their specificity, sensitivity and found at par with OIE peptides and their DIVA potential studies after the dose optimization studies in cattle and buffaloes have also been completed. BCG immunogenicity study has been completed in calves at TANUVAS and is being carried out at LUVAS in buffaloes.
  7. Newcastle Disease Vaccine (NDV): Transcription cassette consisting of VP1 and VP2 genes of chicken anaemia virus (CAV) along with the regulatory signals of NDV has been constructed and introduced within the P and M genes of NDV strain R2B. Recombinant NDV containing the VP1 and VP2 genes of CAV has been rescued from vero cells, and grown in 9-11 days old embryonated chicken eggs to develop genetically engineered vaccine against Newcastle disease.

 

Lateral flow assay kit for diagnosis of Immune mediated haemolytic anaemia in Dogs

CPV2b Vaccine for Canine Parvo nteritis in dogs

  

[Technologies/products developed /transferred for commercialization]

 

Success story Animal Biotechnology.docx

 

Products and Technologies

S.No Title Details
1 Animal Health Products View pdf (452 KB)
2 TRPVB Technologies & Services commercialized View pdf (615.03 KB)

 

Contacts Concerned Officer for more Information

Programme Head Dr. Nitin Kumar Jain, Scientist F
Email nitink[dot]jain[at]nic[dot]in
Phone No. 011-24365972
Programme Officer Dr. Addanki Vamsi krishna, Scientist E
Email vk[dot]addanki[at]nic[dot]in
Phone No. 011-24363725
Attached Officer Dr. Lokesh Kumar Narnoliya, Scientist C
Email lokesh[dot]narnoliya[at]dbt[dot]nic[dot]in
Phone No. 011-24360295